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anti fade vestashield mounting medium  (Vector Laboratories)


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    Structured Review

    Vector Laboratories anti fade vestashield mounting medium
    Anti Fade Vestashield Mounting Medium, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 21861 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+fade+vestashield+mounting+medium/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/pmc03285632-72-116-120
    Average 98 stars, based on 21861 article reviews
    anti fade vestashield mounting medium - by Bioz Stars, 2026-09
    98/100 stars

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    Incubation:

    Article Title: Pathobiological Implications of the Expression of EGFR, pAkt, NF-κB and MIC-1 in Prostate Cancer Stem Cells and Their Progenies
    Article Snippet: The cells were blocked in 10% goat serum for 30 min and incubated with phycoerythrin-conjugated monoclonal anti-CD133/2 antibody (293C3), mouse monoclonal anti-CD44 (HCAM, F-4) antibody, rabbit polyclonal anti-ABCG2 antibody (B-25), rabbit polyclonal anti-EGFR antibody (1105), goat polyclonal anti-Tyr 1173 -pEGFR antibody (1173), rabbit monoclonal anti-pAkt antibody (D9E), rabbit polyclonal anti-NF-κB antibody (C-20), rabbit polyclonal anti-MIC-1 antibody or mouse monoclonal anti-β-actin antibody (clone AC-15) diluted in PBS for 1 h at room temperature. .. After three washes with PBS, the cells were then incubated with fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse, FITC-conjugated donkey anti-goat and/or Texas red-conjugated goat anti-rabbit secondary antibody (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) for 1 h. In addition, the SP cells treated with different cytotoxic agents during 4 days were stained with MitoTracker Red CMXRos in humidified chamber at 37°C in the dark for 30 min prior to the fixation and staining of SP cells with mouse monoclonal anti-cytochrome c antibody for 1 h followed by an incubation with FITC-conjugated goat anti-mouse for 1 h. Then, all of PC cells were washed again with PBS, nuclei counterstained with diamidino-2-phenylindole (DAPI) and mounted on glass slides in anti-fade Vestashield mounting medium (Vector Laboratories, Burlingame, CA). .. Immunofluorescence staining was observed under a confocal laser scanning microscope (LSM 410, Zeiss, Gottingen, Germany).

    Article Title: Cytotoxic effects induced by docetaxel, gefitinib and cyclopamine on side population and non-side population cell fractions from human invasive prostate cancer cells
    Article Snippet: .. After three washes with PBS, the cells were then incubated with fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse, FITC-conjugated donkey anti-goat and/or Texas red-conjugated goat anti-rabbit secondary antibody (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) for 1 h. Cells were washed again with PBS, nuclei counterstained with DAPI (diamidino-2-phenylindole) and mounted on glass slides in anti-fade Vestashield mounting medium (Vector Laboratories, Burlingame, CA). .. Immunofluorescence staining was observed under a confocal laser scanning microscope (LSM 410, Zeiss, Gottingen, Germany).

    Staining:

    Article Title: Pathobiological Implications of the Expression of EGFR, pAkt, NF-κB and MIC-1 in Prostate Cancer Stem Cells and Their Progenies
    Article Snippet: The cells were blocked in 10% goat serum for 30 min and incubated with phycoerythrin-conjugated monoclonal anti-CD133/2 antibody (293C3), mouse monoclonal anti-CD44 (HCAM, F-4) antibody, rabbit polyclonal anti-ABCG2 antibody (B-25), rabbit polyclonal anti-EGFR antibody (1105), goat polyclonal anti-Tyr 1173 -pEGFR antibody (1173), rabbit monoclonal anti-pAkt antibody (D9E), rabbit polyclonal anti-NF-κB antibody (C-20), rabbit polyclonal anti-MIC-1 antibody or mouse monoclonal anti-β-actin antibody (clone AC-15) diluted in PBS for 1 h at room temperature. .. After three washes with PBS, the cells were then incubated with fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse, FITC-conjugated donkey anti-goat and/or Texas red-conjugated goat anti-rabbit secondary antibody (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) for 1 h. In addition, the SP cells treated with different cytotoxic agents during 4 days were stained with MitoTracker Red CMXRos in humidified chamber at 37°C in the dark for 30 min prior to the fixation and staining of SP cells with mouse monoclonal anti-cytochrome c antibody for 1 h followed by an incubation with FITC-conjugated goat anti-mouse for 1 h. Then, all of PC cells were washed again with PBS, nuclei counterstained with diamidino-2-phenylindole (DAPI) and mounted on glass slides in anti-fade Vestashield mounting medium (Vector Laboratories, Burlingame, CA). .. Immunofluorescence staining was observed under a confocal laser scanning microscope (LSM 410, Zeiss, Gottingen, Germany).



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    98
    Vector Laboratories anti fade vestashield mounting medium
    Anti Fade Vestashield Mounting Medium, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+fade+vestashield+mounting+medium/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/pmc03285632-72-116-120
    Average 98 stars, based on 1 article reviews
    anti fade vestashield mounting medium - by Bioz Stars, 2026-09
    98/100 stars
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